For the 30th anniversary of Now 1 in 2013 and the release of Now 100 in 2018, the first Now album was re-compiled and re-issued. Since the release of Now 102, the corresponding Now album from 100 volumes ago has been re-compiled and re-issued on 2-CD sets on the same day. Due to licensing issues, these re-compiled versions often use alternative mixes such as album versions and sometimes tracks are missing where it has not been possible to license the song.
The original Now Dance series began in 1985 and has been released in a variety of titles, formats, and release frequency. Originally, the series captured extended 12" mixes of dance hits of the time, but from 1991 onwards, all Now Dance compilations featured 7" edits with only occasional extended versions or mixes included. The first two volumes were not released on compact disc, and all subsequent releases were either single, double, or triple disc sets. Unlike the main series, Now Dance were originally titled and numbered by their year of release (i.e. Now Dance '89). The frequency in which they were released also varied from year to year; initially, and most frequently across the series, there would be one Now Dance volume per year, but in 1994 for example, there were five volumes released, and in 1996, there weren't any Now Dance compilations released. Towards the end of the series, 3-CD digipak sets were issued and often under The Very Best of Now Dance title. In 2010, the long-running title was retired, and all subsequent dance-themed Now collections have been issued under the Special Editions series. In 2021, the original concept of Now Dance was revived with a new series of compilations containing 12" remixes released under the title, Now That's What I Call Music 12" 80s.
The 4-CD series follows the same general format of the original Special Editions series, with genre, era, and decade collections, but over four compact discs (there is also an abridged vinyl version of Punk and New Wave and Rock), meaning they contain more tracks than the original special editions, but fewer than the Now 100 Hits, which preceded this series. They are only issued in cardboard gatefold wallets, with a slot cut into each sleeve which stores the discs.
The Yearbook series are 4-CD and 3-LP vinyl sets, each representing a year of music, which launched in June 2021. Each release has a tie-in block of programming on Now 80s. Each release is issued as a limited edition mini-hardback book, with the CDs in sleeves forming the pages of the 'yearbook' which contains an introductory overview of the year, original 7" single artwork, trivia, chart positions and a quiz. The limited edition 3-LP version is released on bright coloured vinyl. A standard 4-CD version is also issued in a gatefold "wallet" design, which retails cheaper than the mini-hardback books. The first collection focuses on hits from 1983, the birth year of the Now That's What I Call Music series. The year 1984 followed, but after this, the series rewound its year of focus, issuing collections that went from 1982 and backwards into the late 1970s. A triple-CD only release, Yearbook Extra: The Collectors Edition, is issued a few weeks later; these include lesser known tracks and more songs by big artists included on the main album and they are released only in standard gatefold wallet packaging. A further release, The Final Chapter, a deluxe 4-CD and 3-LP set, was issued in December 2022, rounding off the Yearbook years 1980 to 1984. The same month, a deluxe 5-LP boxset, Now Yearbook '80 - '84: Vinyl Extra, was released. This was the first time tracks from the Extra CD series had been issued on the vinyl format.
A lila (lit., night) generally lasts from sunsetuntil dawn, and in some cases a full derdeba may stretchover several nights. The length depends in part on the mood ofthe participants, the number of spirits who must be propitiated,the seriousness of each case, and the resources of the sponsors.Some sections may get little more than a perfunctory run-through,but all seven families of spirits must be acknowledged in themusic.
Selective alignment, first introduced by the --validateMappings flagin salmon, and now the default mapping strategy (in version 1.0.0forward), is a major feature enhancement introduced in recent versions ofsalmon. When salmon is run with selective alignment, it adopts aconsiderably more sensitive scheme that we have developed for finding thepotential mapping loci of a read, and score potential mapping loci usingthe chaining algorithm introduced in minimap2 5. It scores andvalidates these mappings using the score-only, SIMD, dynamic programmingalgorithm of ksw2 6. Finally, we recommend using selectivealignment with a decoy-aware transcriptome, to mitigate potentialspurious mapping of reads that actually arise from some unannotatedgenomic locus that is sequence-similar to an annotated transcriptome. Theselective-alignment algorithm, the use of a decoy-aware transcriptome, andthe influence of running salmon with different mapping and alignmentstrategies is covered in detail in the paper Alignment and mapping methodology influence transcript abundance estimation.
Note: This sequence-specific bias model is substantially differentfrom the bias-correction methodology that was used in Salmon versionsprior to 0.6.0. This model specifically accounts forsequence-specific bias, and should not be prone to the over-fittingproblem that was sometimes observed using the previous bias-correctionmethodology.
Salmon, has the user provide a description of the type of sequencinglibrary from which the reads come, and this contains information aboute.g. the relative orientation of paired-end reads. As of version0.7.0, Salmon also has the ability to automatically infer (i.e. guess)the library type based on how the first few thousand reads map to thetranscriptome. To allow Salmon to automatically infer the librarytype, simply provide -l A or --libType A to Salmon. Even if youallow Salmon to infer the library type for you, you should still readthe section below, so that you can interpret how Salmon reports thelibrary type it discovers.
Nowak RG, Ambulos NP, Schumaker LM, Mathias TJ, White RA, Troyer J, Wells D, Charurat ME, Bentzen SM, Cullen KJ. Genotyping of high-risk anal human papillomavirus (HPV): ion torrent-next generation sequencing vs. linear array.Virol J. 2017 Jun 13;14(1):112. doi: 10.1186/s12985-017-0771-z. PubMed PMID:28610586; PubMed Central PMCID: PMC5470268.
Jealousy, with all its venom, and all its misery, entered my breast. Now I shed a torrent of tears, to think that I should never call her mine; and, anon, I imprecated a thousand  curses on her inconstancy. Yet, still I must stir the fires of the alchymist, still attend on the changes of his unintelligible medicines.
Cornelius had watched for three days and nights, nor closed his eyes. The progress of his alembics was slower than he expected: in spite of his anxiety, sleep weighed upon his eyelids. Again and again he threw off drowsiness with more than human energy; again and again it stole away his senses. He eyed his crucibles wistfully. "Not ready yet," he murmured; "will another night pass before the work is accomplished? Winzy, you are vigilant--you are faithful--you have slept, my boy--you slept last night. Look at that glass vessel. The liquid it contains is of a soft rose-colour: the moment it begins to change its hue, awaken me--till then I may close my eyes. First, it will turn white, and then emit golden flashes; but wait not till then; when the rose-colour fades, rouse me." I scarcely heard the last words, muttered, as they were, in sleep. Even then he did not quite yield to nature. "Winzy, my boy," he again said, "do not touch the vessel--do not put it to your lips; it is a philter--a philter to cure love; you would not cease to love your Bertha--beware to drink!"
The hours danced away. The philosopher, secure that he had once succeeded, and believing that he might again, began to concoct the same medicine once more. He was shut up with his books and drugs, and I had a holiday. I dressed myself with care; I looked in an old but polished shield, which served me for a mirror; methought my good looks had wonderfully improved. I hurried beyond the precincts of the town, joy in my soul, the beauty of heaven and earth around me. I turned my steps towards the castle--I could look on its lofty turrets with lightness of heart, for I was cured of love. My Bertha saw me afar off, as I came up the avenue. I know not what sudden impulse animated her bosom, but at the sight, she sprung with a light fawn-like bound down the marble steps, and was hastening towards me. But I had been perceived by another person. The old high-born hag, who called herself her protectress, and was her tyrant, had seen me, also; she hobbled, panting, up the terrace; a page, as ugly as herself, held up her train, and fanned her as she hurried along, and stopped my fair girl with a "How, now, my bold mistress? whither so fast? Back to your cage--hawks are abroad!"
Five years afterwards I was suddenly summoned to the bedside of the dying Cornelius. He had sent for me in haste, conjuring my instant presence. I found him stretched on his pallet, enfeebled even to death; all of life that yet remained animated his piercing eyes, and they were fixed on a glass vessel, full of a roseate liquid.
I continued to entertain this belief for many years. Sometimes a thought stole across me--Was the alchymist indeed deceived? But my habitual credence was, that I should meet the fate of all the children of Adam at my appointed time--a little late, but still at a natural age. Yet it was certain that I retained a wonderfully youthful look. I was laughed at for my vanity in consulting the mirror so often, but I consulted it in vain--my brow was untrenched--my cheeks--my eyes--my whole person continued as untarnished as in my twentieth year. 2b1af7f3a8